Journal: PLoS Genetics

Article Title: Function of multiple sclerosis-protective HLA class I alleles revealed by genome-wide protein-quantitative trait loci mapping of interferon signalling

doi: 10.1371/journal.pgen.1009199

Figure Lengend Snippet: (A) A schematic picture of the IFNAR2 isoforms. (B) IFNAR2bc , IFNAR2b and IFNAR2c mRNA levels in isolated B cells determined using qRT-PCR. mRNA levels are normalized to the expression of the reference gene RPL13A and expressed as arbitrary units (AU) ( n = 47, simple linear regression using the sum of MS-protective class I alleles (HLAsum) as independent variable). (C) Correlation between IFNAR2bc mRNA levels and IFNAR2 surface receptor levels determined using flow cytometry ( n = 47). Data are binned into 0, 1 and ≥2 HLAsum. p-values and Pearson’s correlation coefficient (r) from linear regressions of each group are shown in coloured text. p-values from multiple linear regressions of the combined data using HLAsum (continuous variable) and mRNA values as independent variables in the full model are denoted in black. (D) Heat-maps comparing the correlation between IFNAR2 levels and IFN-α induced pSTAT1 (top) or pSTAT4 (bottom) in indicated cell-types with and without including HLAsum as a covariate in the full model ( n = 303). (E-G) Correlation between IFNAR2 protein levels and IFN-α (2000 IU/ml) induced pSTAT1 (E), pSTAT4 (F) and CXCL10 (G) in B cells. p-values as described in (C), ( n = 303). Boxplots show median, IQR and range. gMFI = geometric mean fluorescence intensity.

Article Snippet: The IFNAR2 mAb REA124 was validated by transfection of HEK293T cells with siRNA targeting IFNAR2 ((dTdT-)GCACCATAGTGACACTGAA-dTdT), and plasmids encoding IFNAR2b or IFNAR2c (#RC201212 and #RC238664, Origene) ( ).

Techniques: Isolation, Quantitative RT-PCR, Expressing, Flow Cytometry, Fluorescence

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Main immunohistochemical findings in the study groups

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Additional immunohistochemical findings (intensity score in alveolar walls and bronchiolar epithelium)

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining